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Mouse ATP-citrate synthase (ACLY) ELISA kit (0.313-20 ng/mL, 0.188 ng/mL)
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Cat. No.:
OB0525WXX-100
Synonyms:
Mouse ACLY ELISA kit; ATP-citrate synthase (ACLY); Mouse ATP-citrate synthase; ACLY ELISA kit; Mouse ACLY; EC 2.3.3.8
Size:
48T; 96T
Specification
Description:
Mouse ATP-citrate synthase (ACLY) ELISA kit (0.313-20 ng/mL, 0.188 ng/mL) is a ready-to-use ELISA kit for analyzing mouse ACLY levels with a detection range of 0.313-20 ng/mL and a sensitivity of 0.188 ng/mL.
Background:
ACLY is the major enzyme responsible for the synthesis of cytoplasmic acetyl-CoA in many tissues. The product it catalyzes, acetyl-CoA, is used in several important biosynthetic pathways, including cholesterogenesis and lipogenesis.
Formula Weight:
91,099 Da
Assay Principle:
The kit is based on a sandwich ELISA. The capture antibody is pre-coated on a 96-well plate. A biotin-conjugated antibody is then used as the detection antibody. Standards, test samples, and biotin-conjugated detection antibodies are then added to the wells and washed with buffer. HRP-streptavidin is added, and the unbound conjugate is washed away with washing solution. TMB substrate is used to visualize the HRP enzyme reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The density of the yellow color is proportional to the amount of target sample captured in the plate. The concentration of the target can be calculated by reading the absorbance at 450nm with a microplate reader.
ELISA Type:
Double antibody sandwich
Assay Target:
ACLY
Target Species:
Mouse
Research Area:
Carbohydrate metabolism; Lipid metabolism; Biosynthesis; Obesity
Sample Type:
Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples
Detection Range:
0.313-20 ng/mL
Sensitivity:
0.188 ng/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<10%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Mouse ATP-citrate synthase (ACLY) ELISA kit (0.313-20 ng/mL, 0.188 ng/mL) is designed for the in vitro quantitative analysis of ACLY levels in mouse serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
Storage:
Store at -20°C (microplate, standard) and 2-8°C (other reagents).