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Mouse agouti-related protein (AGRP) ELISA kit (15.6-1000 pg/mL; 5 pg/mL)
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Cat. No.:
OB0525WXX-389
Synonyms:
Mouse AGRP ELISA kit; Mouse agouti-related protein (AGRP); Mouse agouti-related protein; Agouti-related protein (AGRP); Mouse AGRP; AGRP ELISA kit
Size:
48T; 96T
Specification
Description:
Mouse agouti-related protein (AGRP) ELISA kit (15.6-1000 pg/mL; 5 pg/mL) is a ready-to-use ELISA kit for analyzing mouse AGRP levels with a detection range of 15.6-1000 pg/mL and a sensitivity of 5 pg/mL.
Background:
AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.
Formula Weight:
14,440 Da
Assay Principle:
This assay utilizes a double sandwich ELISA technique, and the ELISA kit provided is typical. The pre-coated antibody is a mouse monoclonal antibody to AGRP, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and biotin-labeled antibodies are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells; the reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue when catalyzed by peroxidase and finally turns yellow in the presence of acid. The color is positively correlated with the detection factor in the sample.
ELISA Type:
Sandwich
Assay Target:
AGRP
Target Species:
Mouse
Research Area:
Hormone research; Eating behavior; Signaling pathway; Obesity
Sample Type:
Mouse serum; Plasma; Cell culture supernatant; Organizations in the natural and recombinant AGRP concentration
Detection Range:
15.6-1000 pg/mL
Sensitivity:
5 pg/mL
Precision (Intra-assay):
CV≤8%
Precision (Inter-assay):
CV≤12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Mouse agouti-related protein (AGRP) ELISA kit (15.6-1000 pg/mL, 5 pg/mL) is designed for the in vitro quantitative analysis of AGRP levels in mouse serum, plasma, cell culture supernatant, organizations in the natural and recombinant AGRP concentration samples.
Storage:
Store at 2-8°C.