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Mouse agouti-related protein (AGRP) ELISA kit (15.6-1000 pg/mL, 3.9 pg/mL)
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Cat. No.:
OB0525WXX-353
Synonyms:
Mouse AGRP ELISA kit; Mouse agouti-related protein (AGRP); Mouse agouti-related protein; Agouti-related protein (AGRP); Mouse AGRP; AGRP ELISA kit
Size:
24T; 48T; 96T
Specification
Description:
Mouse agouti-related protein (AGRP) ELISA kit (15.6-1000 pg/mL, 3.9 pg/mL) is a ready-to-use ELISA kit for analyzing mouse AGRP levels with a detection range of 15.6-1000 pg/mL and a sensitivity of 3.9 pg/mL.
Background:
AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.
Formula Weight:
14,432 Da
Assay Principle:
The assay utilizes a quantitative sandwich enzyme immunoassay technique. An antibody specific for AGRP is pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any AGRP is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific to AGRP is added to the wells. After washing, avidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of AGRP bound in the first step. The color development is stopped, and the color intensity is measured.
ELISA Type:
Sandwich
Assay Target:
AGRP
Target Species:
Mouse
Research Area:
Hormone research; Eating behavior; Signaling pathway; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates
Detection Range:
15.6-1000 pg/mL
Sensitivity:
3.9 pg/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<10%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Mouse agouti-related protein (AGRP) ELISA kit (15.6-1000 pg/mL, 3.9 pg/mL) is designed for the in vitro quantitative analysis of AGRP levels in mouse serum, plasma, and tissue homogenate samples.
Storage:
Store at 2-8°C.