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Mouse adrenocorticotropic hormone (ACTH) ELISA kit (5-600 ng/L)
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Cat. No.:
OB0525WXX-185
Synonyms:
Mouse ACTH ELISA kit; Mouse adrenocorticotropic hormone (ACTH); Mouse adrenocorticotropic hormone; Adrenocorticotropic hormone (ACTH); ACTH ELISA kit; Mouse ACTH
Size:
48T; 96T
Specification
Description:
Mouse adrenocorticotropic hormone (ACTH) ELISA kit (5-600 ng/L) is a ready-to-use ELISA kit for analyzing mouse ACTH levels with a detection range of 5-600 ng/L and a sensitivity of 3.13 ng/L.
Background:
ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.
Formula Weight:
26,707 Da
Assay Principle:
The plates are pre-coated with mouse ACTH antibody. ACTH added to the sample binds to the antibody coated on the wells. Biotinylated mouse ACTH antibody is then added and binds to ACTH in the sample. Streptavidin-HRP is then added and binds to the biotinylated ACTH antibody. After incubation, unbound streptavidin-HRP is washed out. The substrate solution is then added, and the color is developed in proportion to the mouse ACTH content. The reaction is terminated by the addition of an acidic termination solution, and the absorbance is measured at 450 nm.
ELISA Type:
Quantitative sandwich
Assay Target:
ACTH
Target Species:
Mouse
Research Area:
Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity
Sample Type:
Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates
Detection Range:
5-600 ng/L
Sensitivity:
3.13 ng/L
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<10%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Stability:
6 Months
Applications:
Mouse adrenocorticotropic hormone (ACTH) ELISA kit (5-600 ng/L) is designed for the in vitro quantitative analysis of ACTH levels in mouse serum, plasma, cell culture supernates, cell lysates, and tissue homogenate samples.
Storage:
Store at 2-8°C.