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Mouse adrenocorticotropic hormone (ACTH) ELISA kit (15.6-1000 pg/mL, 6.39 pg/mL)
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Cat. No.:
OB0525WXX-197
Synonyms:
Mouse ACTH ELISA kit; Mouse adrenocorticotropic hormone (ACTH); Mouse adrenocorticotropic hormone; Adrenocorticotropic hormone (ACTH); ACTH ELISA kit; Mouse ACTH
Size:
48T; 96T
Specification
Description:
Mouse adrenocorticotropic hormone (ACTH) ELISA kit (15.6-1000 pg/mL, 6.39 pg/mL) is a ready-to-use ELISA kit for analyzing mouse ACTH levels with a detection range of 15.6-1000 pg/mL and a sensitivity of 6.39 pg/mL.
Background:
ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.
Formula Weight:
26,707 Da
Assay Principle:
The microtiter plates provided in this kit are pre-coated with an ACTH-specific antibody. Standards or samples are then added to the appropriate microtiter plate wells along with the ACTH-specific biotin-conjugated antibody preparation. HRP-conjugated avidin is then added to each microtiter plate well and incubated. Upon addition of the TMB substrate solution, only the wells containing ACTH, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of PAPP in the sample is then determined by comparing the OD of the sample to a standard curve.
ELISA Type:
Quantitative sandwich
Assay Target:
ACTH
Target Species:
Mouse
Research Area:
Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity
Sample Type:
Mouse serum; Plasma; Other biological fluids
Detection Range:
15.6-1000 pg/mL
Sensitivity:
6.39 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Mouse adrenocorticotropic hormone (ACTH) ELISA kit (15.6-1000 pg/mL, 6.39 pg/mL) is designed for the in vitro quantitative analysis of ACTH levels in mouse serum, plasma, and other biological fluid samples.
Storage:
Store at 4°C.