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Mouse adrenocorticotropic hormone (ACTH) ELISA kit (0.312-20 ng/mL)
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Cat. No.:
OB0525WXX-180
Synonyms:
Mouse ACTH ELISA kit; Mouse adrenocorticotropic hormone (ACTH); Mouse adrenocorticotropic hormone; Adrenocorticotropic hormone (ACTH); ACTH ELISA kit; Mouse ACTH
Size:
48T; 96T
Specification
Description:
Mouse adrenocorticotropic hormone (ACTH) ELISA kit (0.312-20 ng/mL) is a ready-to-use ELISA kit for analyzing mouse ACTH levels with a detection range of 0.312-20 ng/mL and a sensitivity of 0.126 ng/mL.
Background:
ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.
Formula Weight:
26,707 Da
Assay Principle:
The microtiter plates provided in this kit are pre-coated with specific ACE antibody, and standards or samples are then added to the appropriate microtiter plate wells along with the biotin-conjugated specific ACE antibody preparation. Avidin conjugated to HRP is then added to each microplate well and incubated. Only wells containing ACE, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ACE in the sample is then determined by comparing the OD of the sample with the standard curve.
ELISA Type:
Quantitative sandwich
Assay Target:
ACTH
Target Species:
Mouse
Research Area:
Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity
Sample Type:
Mouse serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids
Detection Range:
0.312-20 ng/mL
Sensitivity:
0.126 ng/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Mouse adrenocorticotropic hormone (ACTH) ELISA kit (0.312-20 ng/mL) is designed for the in vitro quantitative analysis of ACTH levels in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples.
Storage:
Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).