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Mouse adiponectin (ADP) ELISA kit (31.25-2000 pg/mL, 14.5 pg/mL)
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Cat. No.:
OB0525WXX-265
Synonyms:
Mouse ADP ELISA kit; Mouse adiponectin (ADP); Mouse adiponectin; Adiponectin (ADP); Mouse ADP; ADP ELISA kit
Size:
48T; 96T
Specification
Description:
Mouse adiponectin (ADP) ELISA kit (31.25-2000 pg/mL, 14.5 pg/mL) is a ready-to-use ELISA kit for analyzing mouse ADP levels with a detection range of 31.25-2000 pg/mL and a sensitivity of 14.5 pg/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,809 Da
Assay Principle:
The microtiter plate provided in this kit is pre-coated with a specific ADP antibody. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for ADP. Avidin conjugated to HRP is then added to each microtiter plate well and incubated. Only wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The OD of the sample is then compared to a standard curve to determine the concentration of ADP in the sample.
ELISA Type:
Sandwich
Assay Target:
ADP
Target Species:
Mouse
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids
Detection Range:
31.25-2000 pg/mL
Sensitivity:
14.5 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Mouse adiponectin (ADP) ELISA kit (31.25-2000 pg/mL, 14.5 pg/mL) is designed for the in vitro quantitative analysis of ADP levels in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples.
Storage:
Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).