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Mouse adiponectin (ADP) ELISA kit (0.312-20 ng/mL, <10 pg/mL)
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Cat. No.:
OB0525WXX-220
Synonyms:
Mouse ADP ELISA kit; Mouse adiponectin (ADP); Mouse adiponectin; Adiponectin (ADP); Mouse ADP; ADP ELISA kit
Size:
96T
Specification
Description:
Mouse adiponectin (ADP) ELISA kit (0.312-20 ng/mL, <10 pg/mL) is a ready-to-use ELISA kit for analyzing mouse ADP levels with a detection range of 0.312-20 ng/mL. Its sensitivity is less than 10 pg/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,809 Da
Assay Principle:
The detection antibody is a biotinylated antibody specific for ADP. The capture antibody is a rat monoclonal antibody, and the detection antibody is a goat polyclonal antibody. To detect mouse ADP, standards and samples are added to the wells, followed by biotinylated detection antibody. The wells are washed with PBS or TBS buffer, and ABC-HRP is added. Unbound ABC-HRP is washed with PBS or TBS buffer, and TMB is added. TMB is an HRP substrate that catalyzes the production of a blue product, which turns yellow upon addition of an acidic termination solution. The absorbance of the yellow product at 450 nm is linearly proportional to the mouse ADP in the sample. The absorbance of the yellow product in each well is read using a plate reader, and the readings from the sample wells are compared to a standard curve to determine the concentration of mouse ADP in the sample.
Assay Target:
ADP
Target Species:
Mouse
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Cell culture supernatants; serum; Plasma (Heparin; edta); Urine
Detection Range:
0.312-20 ng/mL
Sensitivity:
<10 pg/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Stability:
12 Months
Applications:
Mouse adiponectin (ADP) ELISA kit (0.312-20 ng/mL, <10 pg/mL) is designed for the in vitro quantitative analysis of ADP levels in mouse cell culture supernatants, serum, plasma (heparin, edta), and urine samples.
Storage:
Store at 2-8°C.