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Mouse acetyl-CoA carboxylase (ACAC) ELISA kit
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Cat. No.:
OB0525WXX-089
Synonyms:
Mouse ACAC ELISA kit; Acetyl-CoA carboxylase (ACAC); ACAC ELISA kit; Mouse ACAC; Mouse acetyl-CoA carboxylase; EC 6.4.1.2
Size:
48T; 96T
Specification
Description:
Mouse acetyl-CoA carboxylase (ACAC) ELISA kit is a ready-to-use ELISA kit for analyzing mouse ACAC levels with a detection range of 0.312-20 ng/mL and a sensitivity of 0.06 ng/mL. It is an effective dynamic research tool.
Background:
ACAC (also known as ACC) is the enzyme that catalyzes the carboxylation of acetyl coenzyme A to produce malonyl coenzyme A, which is involved in fatty acid biosynthesis and β-oxidation. It is a potential target for the treatment of obesity and related diseases.
Formula Weight:
265,303 Da
Assay Principle:
The double sandwich ELISA technique is used in this experiment. The pre-coated antibody is a mouse ABP monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and biotin-labeled antibodies are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells; the reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue when catalyzed by peroxidase, and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.
ELISA Type:
Sandwich
Assay Target:
ACAC
Target Species:
Mouse
Research Area:
Metabolites; Fatty acid biosynthesis; Obesity
Sample Type:
Mouse serum; Plasma; Cell culture supernatant; Organizations in the natural and recombinant ACAC concentration
Detection Range:
0.312-20 ng/mL
Sensitivity:
0.06 ng/mL
Precision (Intra-assay):
CV≤8%
Precision (Inter-assay):
CV≤12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Mouse acetyl-CoA carboxylase (ACAC) ELISA kit is designed for the in vitro quantitative analysis of ACAC levels in mouse serum, plasma, cell culture supernatant, and natural and recombinant ACAC concentration samples.
Storage:
Store at 2-8°C.