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Mouse acetyl-CoA carboxylase 1 (ACACA) ELISA kit
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Cat. No.:
OB0525WXX-013
Synonyms:
Mouse ACACA ELISA kit; ACACA ELISA kit; Acetyl-CoA carboxylase 1 (ACACA); ACACA; Mouse ACACA; EC 6.3.4.14
Size:
48T; 96T
Specification
Description:
Mouse acetyl-CoA carboxylase 1 (ACACA) ELISA kit is a ready-to-use ELISA kit for analyzing mouse ACACA levels with high sensitivity.
Background:
ACACA (also known as ACC1) is the first rate-limiting enzyme in fatty acid synthesis and is primarily responsible for converting cytoplasmic acetyl-CoA to malonyl-CoA. It plays a central role in fatty acid synthesis and is a promising therapeutic target for the treatment of obesity, cancer, metabolic diseases, and other disorders.
Formula Weight:
269,999 Da
Assay Principle:
The assay utilizes a two-site sandwich ELISA to quantify ACACA in the sample. An antibody specific for ACACA has been pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any ACACA present is bound by the immobilized antibody. After removing unbound material, a biotin-conjugated antibody specific for ACACA is added to the wells. After washing, streptavidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color is developed in proportion to the amount of ACACA bound in the first step. The color development is stopped, and the intensity of the color is measured.
ELISA Type:
Sandwich
Assay Target:
ACACA
Target Species:
Mouse
Research Area:
Metabolites; Fatty acid biosynthesis; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Sample Volume:
1-200 μL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Mouse acetyl-CoA carboxylase 1 (ACACA) ELISA kit is designed for the in vitro quantitative analysis of ACACA levels in mouse serum, plasma, and other biological fluid samples.
Storage:
Store at 2-8°C.