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Malate dehydrogenase (MDH) activity assay kit (Colorimetric)
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Cat. No.:
OB0525WXX-419
Synonyms:
Malate dehydrogenase; MDH assay kit; Malate dehydrogenase (MDH); MDH
Size:
100T
Specification
Description:
Malate dehydrogenase (MDH) activity assay kit (Colorimetric) is an enzyme assay kit for the rapid and sensitive detection of MDH. The detection limit is 0.5 U/L.
Background:
Malate dehydrogenase (MDH) is an enzyme that reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are two isoenzymes in eukaryotic cells: MDH1 and MDH2. MDH1 is found in the cytoplasm and plays a key role in the malate-aspartate shuttle that transports malate into the mitochondria. MDH2 is a mitochondrial enzyme that participates in the TCA cycle and reversibly converts L-malate to oxaloacetate.
Assay Type:
Activity assay
Assay Principle:
The tetrazolium salt MTT is reduced to reduced MTT in the NADH-coupled enzymatic reaction, which shows a maximum absorption peak at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Assay Target:
MDH
Target Species:
Universal
Research Area:
Ferroptosis; TCA cycle; Urea; Enzyme activity; Obesity research
Sample Type:
Plasma; Serum; Erythrocytes; Tissue; Culture media)
Kit Components:
Assay buffer; Enzyme A; NAD/MTT; Enzyme B; Substrate; Calibrator
Detection Limit:
0.5 U/L
Reaction Time:
30 min
Stability:
6 months
Applications:
Malate dehydrogenase (MDH) activity assay kit (Colorimetric) is used for the quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism.
Storage:
Store at 2-8°C.