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LDH cytotoxicity assay kit
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Cat. No.:
OB0125WXX-061
Synonyms:
Cell cytotoxicity assay kit
Size:
960T
Specification
Description:
LDH cytotoxicity assay kit is a cytotoxicity assay kit that uses a colorimetric method to measure and monitor cytotoxicity. Researchers can culture cells and then treat them with compounds or agents that affect cell viability.
Background:
Measurement and monitoring of cytotoxicity are essential in cell-based studies. This technique allows optimization of cell culture conditions, and cytotoxicity of anti-obesity drugs in toxicology testing, toxicity of therapeutic chemicals in drug screening, and cell-mediated cytotoxicity can be assessed by this assay-based approach. Cytotoxicity characteristics include loss of cellular metabolic activity or loss of cell membrane integrity.
Assay Principle:
In the assay, cells may be cultured and then treated with compounds or agents that affect cell viability. Upon cell death, lactate dehydrogenase (LDH), a soluble enzyme in the cytoplasm, is released into the growth medium. The growth medium is then transferred to another plate, and the released LDH is detected with a cytotoxicity reagent. In the presence of a lactate substrate (included in the LDH cytotoxicity reagent), LDH converts lactate to pyruvate and produces nicotinamide adenine dinucleotide (NADH). The WST-1 molecule, also present in the LDH cytotoxicity reagent, is converted from WST-1 to the orange-colored garnetoside form. Increased cytotoxicity is accompanied by increased LDH release and increased colorimetric signal.
Assay Target:
LDH cytotoxicity
Target Species:
Universal
Research Area:
Cell-based research; Obesity
Sample Type:
Mammalian cell
Kit Components:
LDH cytotoxicity assay reagent; Triton X-100 solution
Applications:
LDH cytotoxicity assay kit is used to detect cytotoxicity in mammalian cells. The assay principle is basic and is used in most eukaryotic cell lines, including adherent and non-adherent cells and some tissues, depending on the level of LDH expression.
Storage:
Store at -20°C (LDH cytotoxicity assay reagent) and 4°C (others).