Products
Online Inquiry
L-Lactic acid/lactate assay kit (Colorimetric)
Inquiry
Cat. No.:
OB0525WXX-421
Synonyms:
L-Lactic acid/lactate assay kit; L-Lactic acid/lactate
Size:
96T
Specification
Description:
L-Lactic acid/lactate assay kit (Colorimetric) is a metabolic assay kit for the rapid and sensitive detection of L-Lactic acid/lactate in the range of 0.12-7.0 mmol/L.
Background:
Lactic acid is an intermediate product of glucose metabolism in the body and is produced mainly by red blood cells, rhabdomyosin, and brain tissue. The concentration of lactic acid in the blood depends mainly on the rate of synthesis and metabolism in the liver and kidneys. The bidirectional conversion of lactate and pyruvate is regulated by lactate dehydrogenase (LDH).
Assay Type:
Quantitative assay
Assay Principle:
LDH catalyzes the reaction of lactic acid and NAD+ to form pyruvic acid and NADH, respectively, using NAD+ as the H+ receptor, during which NBT is reduced to a purple compound. The concentration of lactate can be calculated by measuring the OD at 530 nm.
Assay Target:
L-Lactic acid/lactate
Target Species:
Universal
Research Area:
Food safety and analysis; Obesity research; Glycolysis and carbohydrates
Sample Type:
Serum; Plasma; Cell culture supernatant; Tissue; Cells
Kit Components:
Buffer solution; Enzyme stock solution; Chromogenic agent; Stop solution; Standard (10 mmol/L); Microplate; Plate sealer
Detection Range:
0.12-7.0 mmol/L
Sensitivity:
0.10 mmol/L
Precision (Intra-assay):
0.035
Precision (Inter-assay):
0.014
Reaction Time:
30 min
Recovery:
1.05
Stability:
6 Months
Applications:
L-Lactic acid/lactate assay kit (Colorimetric) is used to measure L-lactic acid (LA) content in serum (plasma), tissue, cells, and culture supernatant samples.
Storage:
Store at 2-8°C.