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L-Lactate assay kit (Fluorometric)
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Cat. No.:
OB0525WXX-422
Synonyms:
L-Lactate assay kit; L-Lactate
Size:
100T
Specification
Description:
L-Lactate assay kit (Fluorometric) is a metabolic assay kit for the rapid and sensitive detection of L-lactate. The detection limit is 1 μM.
Background:
Under hypoxic or anaerobic conditions, lactate is produced by lactate dehydrogenase (LDH). Therefore, monitoring lactate levels is a good indicator of the balance between tissue oxygen demand and utilization, and is useful in the study of cellular and animal physiology.
Assay Type:
Quantitative assay
Assay Principle:
Based on the lactate oxidation reaction catalyzed by lactate dehydrogenase, the NADH formed in the reaction reduces the probe to a highly fluorescent product. The fluorescence intensity of this product is measured at Ex/Em = 530/585 nm and is proportional to the concentration of lactic acid in the sample.
Assay Target:
L-Lactate
Target Species:
Universal
Research Area:
TCA cycle; Obesity research; Food safety and analysis
Sample Type:
Serum; Plasma; Cell culture media
Kit Components:
Assay buffer; Enzyme A; NAD solution; Enzyme B; Probe; Standard
Detection Limit:
1 μM
Reaction Time:
60 min
Stability:
6 months
Applications:
L-Lactate assay kit (Fluorometric) is used for quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.
Storage:
Store at 2-8°C.