Products
Online Inquiry
In situ histone H3-K27 tri-methylation assay kit
Inquiry
Cat. No.:
OB0125WXX-050
Synonyms:
Tri-methylation assay kit; Histone H3-K27; Histone methylation assay kit
Size:
96T
Specification
Description:
In situ histone H3-K27 tri-methylation assay kit is an epigenetic test that detects trimethylation of histone H3-K27 in situ under simple, reliable, and consistent conditions.
Background:
Epigenetic activation or inactivation of genes is associated with a variety of diseases. A major mechanism of epigenetic inactivation of genes is the methylation of CpG islands in genomic DNA by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcriptional repression or activation. They transfer 1-3 methyl groups from S-adenosyl-l-methionine to lysine and arginine residues of histones. G9a and polycomb group enzymes are HMTs that catalyze the methylation of histone H3 at lysine 27 (H3-K27) in mammalian cells. Trimethylation of H3-K27 is a facultative heterochromatin mark that promotes the recruitment of polycomb group proteins for gene silencing global H3-K27 trimethylation increased H3-K27 trimethylation has also been found to be associated with several pathological processes. Measurement of histone H3-K27 trimethylation is necessary.
Assay Type:
Methylation assay
Assay Principle:
In this assay, adherent cells are cultured in conventional 96-well microtiter plates. After the experimental treatment, the cells are fixed and permeabilized. Trimethylated H3-K27 is then detected with an anti-trimethylated H3-K27 antibody. The proportion or amount of trimethylated H3-K27 is quantified by the intensity of the HRP-conjugated secondary antibody chromogenicity.
Assay Target:
H3-K27 tri-methylation
Target Species:
Universal
Research Area:
Methylation research; Obesity
Sample Type:
Cell; Tissue
Kit Components:
GK1 (10X wash buffer); GK2 (Permeabilizing buffer); GK3 (Blocking buffer); GK4 (Antibody buffer); GK5 (Capture antibody, 1000 µg/ml); GK6 (Detection antibody, 200 µg/ml); GK7 (Developing solution); GK8 (Stop solution); 30% H2O2 solution; Tri-methyl H3-K27 control (20 µg/ml); 8 well assay strip; Microplate
Applications:
In situ histone H3-K27 tri-methylation assay kit is suitable for the in situ-specific detection of histone H3-K27 trimethylation using cultured adherent cells.
Storage:
Store at -20°C (GK6, tri-methyl H3-K27 control) and 4°C away from light (others).