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Hydroxyl radical antioxidant capacity (HORAC) assay kit
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Cat. No.:
OB0125WXX-057
Synonyms:
HORAC assay kit; Hydroxyl radical antioxidant capacity (HORAC)
Size:
192T
Specification
Description:
Hydroxyl radical antioxidant capacity (HORAC) assay kit is an antioxidant capacity assay kit and provides enough reagents to perform HORAC antioxidant capacity, including blanks, antioxidant standards, and unknown samples.
Background:
Excessive ROS accumulation can lead to cellular damage and has been linked to the development of many diseases such as cancer, diabetes, etc. Products of ROS-induced oxidative stress are widely used to monitor the effects of oxidative stress, but it is also important to assess the antioxidant capacity of biofluids, cells, and extracts. The HORAC assay is a classic tool for measuring the antioxidant capacity of biomolecules in a wide variety of samples.
Assay Principle:
This assay is based on the oxidation of the fluorescent probe by hydroxyl radicals through the process of hydrogen atom transfer (HAT). Hydroxyl radicals are produced by a hydroxyl radical initiator and Fenton's reagent, which quenches the fluorescent probe over time. Antioxidants in the sample prevent hydroxyl radical oxidation of the fluorescent probe until it is depleted. The remaining hydroxyl radicals destroy the fluorescence of the fluorescent probe. The total amount of hydroxyl radical antioxidant activity in the sample is quantified based on the fluorescence decay curve and compared to a gallic acid antioxidant standard curve.
Assay Target:
HORAC
Target Species:
Universal
Research Area:
Antioxidant research; Obesity
Sample Type:
Cell lysate; Plasma; Serum; Tissue homogenates; Food extracts
Kit Components:
96-well microtiter plate; HORAC fluorescein probe (100X); Hydroxyl radical initiator (5X); Gallic acid antioxidant standard; Fenton reagent; Assay diluent (2X)
Applications:
Hydroxyl radical antioxidant capacity (HORAC) assay kit is rapid and reliable for the direct determination of HORAC antioxidant capacity in plasma, serum, cell lysates, tissue homogenates, and food extracts.
Storage:
Store at -20°C (fluorescein probe, antioxidant standard) and 4°C (others).