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Human tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA kit
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Cat. No.:
OB0525WXX-125
Synonyms:
Human TRACP-5b ELISA kit; Human tartrate-resistant acid phosphatase 5b (TRACP-5b); Tartrate-resistant acid phosphatase 5b (TRACP-5b); TRACP-5b ELISA kit; Human TRACP-5b; Human tartrate-resistant acid phosphatase 5b
Size:
48T; 96T
Specification
Description:
Human tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA kit is a ready-to-use ELISA kit for analyzing human TRACP-5b levels. It is an efficient dynamic research tool.
Background:
ACP5 is involved in the dephosphorylation of proteins. Its expression appears to increase in certain pathological states. Mice overexpressing ACP5 have been found to exhibit hyperplastic obesity. There are two phenotypes of this enzyme: 5a and 5b. TRAP 5a binds to preadipocytes and may exert cell number-regulating activity, inducing preadipocyte proliferation/differentiation. TRAP 5b may also induce the proliferation of certain cell types.
Formula Weight:
36,599 Da
Assay Principle:
The assay utilizes a two-site sandwich ELISA to quantify ACP5 in the sample. An antibody specific for ACP5 has been pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any ACP5 present is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific for ACP5 is added to the wells. After washing, streptavidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of ACP5 bound in the initial step. The color development is stopped, and the color intensity is measured.
ELISA Type:
Sandwich
Assay Target:
TRACP-5b
Target Species:
Human
Research Area:
Protein dephosphorylation; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA kit is designed for the in vitro quantitative analysis of TRACP-5b levels in human serum, plasma, and other biological fluid samples.
Storage:
Store at 2-8°C.