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Human tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA kit (0.312-20 mIU/mL)
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Cat. No.:
OB0525WXX-122
Synonyms:
Human TRACP-5b ELISA kit; Human tartrate-resistant acid phosphatase 5b (TRACP-5b); Tartrate-resistant acid phosphatase 5b (TRACP-5b); TRACP-5b ELISA kit; Human TRACP-5b; Human tartrate-resistant acid phosphatase 5b
Size:
24T; 48T; 96T
Specification
Description:
Human tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA kit (0.312-20 mIU/mL) is a ready-to-use ELISA kit for analyzing human TRACP-5b levels with a detection range of 0.312-20 mIU/mL. Its sensitivity is less than 0.078 mIU/mL.
Background:
ACP5 is involved in the dephosphorylation of proteins. Its expression appears to increase in certain pathological states. Mice overexpressing ACP5 have been found to exhibit hyperplastic obesity. There are two phenotypes of this enzyme: 5a and 5b. TRAP 5a binds to preadipocytes and may exert cell number-regulating activity, inducing preadipocyte proliferation/differentiation. TRAP 5b may also induce the proliferation of certain cell types.
Formula Weight:
36,599 Da
Assay Principle:
The assay uses a quantitative sandwich enzyme immunoassay technique. An antibody specific for TRACP-5b is pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any TRACP-5b is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific to TRACP-5b is added to the wells. After washing, avidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of TRACP-5b bound in the initial step. Color development is stopped, and color intensity is measured.
ELISA Type:
Quantitative sandwich
Assay Target:
TRACP-5b
Target Species:
Human
Research Area:
Protein dephosphorylation; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates
Detection Range:
0.312-20 mIU/mL
Sensitivity:
<0.078 mIU/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<10%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA kit (0.312-20 mIU/mL) is designed for the in vitro quantitative analysis of TRACP-5b levels in human serum, plasma, and tissue homogenate samples.
Storage:
Store at 2-8°C.