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Human agouti-related protein (AGRP) ELISA kit (31.2-2000 pg/mL, 12.2 pg/mL)
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Cat. No.:
OB0525WXX-378
Synonyms:
Human AGRP ELISA kit; Human agouti-related protein (AGRP); Human agouti-related protein; Agouti-related protein (AGRP); Human AGRP; AGRP ELISA kit
Size:
24T; 48T; 96T
Specification
Description:
Human agouti-related protein (AGRP) ELISA kit (31.2-2000 pg/mL, 12.2 pg/mL) is a ready-to-use ELISA kit for analyzing human AGRP levels with a detection range of 31.2-2000 pg/mL and a sensitivity of 12.2 pg/mL.
Background:
AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.
Formula Weight:
14,440 Da
Assay Principle:
The detection principle used in this kit is a sandwich enzyme immunoassay. The microtiter plate provided in the kit is pre-coated with an antibody specific for AGRP. Standards or samples are then added to the appropriate wells of the microtiter plate, followed by the addition of a biotin-conjugated antibody specific to AGRP. Avidin conjugated to HRP is then added to each microtiter plate well and incubated. Only wells containing AGRP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of AGRP in the sample can then be determined by comparing the OD of the sample to a standard curve.
ELISA Type:
Double antibody sandwich
Assay Target:
AGRP
Target Species:
Human
Research Area:
Hormone research; Eating behavior; Signaling pathway; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids
Detection Range:
31.2-2000 pg/mL
Sensitivity:
12.2 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human agouti-related protein (AGRP) ELISA kit (31.2-2000 pg/mL, 12.2 pg/mL) is designed for the in vitro quantitative analysis of AGRP levels in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples.
Storage:
Store at 2-8°C.