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Human agouti-related protein (AGRP) ELISA kit (2-600 pg/mL)
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Cat. No.:
OB0525WXX-382
Synonyms:
Human AGRP ELISA kit; Human agouti-related protein (AGRP); Human agouti-related protein; Agouti-related protein (AGRP); Human AGRP; AGRP ELISA kit
Size:
48T; 96T
Specification
Description:
Human agouti-related protein (AGRP) ELISA kit (2-600 pg/mL) is a ready-to-use ELISA kit for analyzing human AGRP levels with a detection range of 2-600 pg/mL and a sensitivity of 1.01 pg/mL.
Background:
AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.
Formula Weight:
14,440 Da
Assay Principle:
The plates are pre-coated with human AGRP antibody. AGRP added to the sample binds to the antibody coated on the wells. Biotinylated human AGRP antibody is then added and binds to AGRP in the sample. Streptavidin-HRP is then added to bind to the biotinylated AGRP antibody. After incubation, unbound streptavidin-HRP is washed out. The substrate solution is then added, and the color is developed in proportion to the human AGRP content. The reaction is terminated by the addition of an acidic termination solution, and the absorbance is measured at 450 nm.
ELISA Type:
Quantitative sandwich
Assay Target:
AGRP
Target Species:
Human
Research Area:
Hormone research; Eating behavior; Signaling pathway; Obesity
Sample Type:
Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates
Detection Range:
2-600 pg/mL
Sensitivity:
1.01 pg/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Stability:
6 Months
Applications:
Human agouti-related protein (AGRP) ELISA kit (2-600 pg/mL) is designed for the in vitro quantitative analysis of AGRP levels in human serum, plasma, cell culture supernates, cell lysates, and tissue homogenate samples.
Storage:
Store at 2-8°C.