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Human agouti-related protein (AGRP) ELISA kit (15.6-1000 pg/mL, ≤5 pg/mL)
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Cat. No.:
OB0525WXX-376
Synonyms:
Human AGRP ELISA kit; Human agouti-related protein (AGRP); Human agouti-related protein; Agouti-related protein (AGRP); Human AGRP; AGRP ELISA kit
Size:
48T; 96T
Specification
Description:
Human agouti-related protein (AGRP) ELISA kit (15.6-1000 pg/mL, ≤5 pg/mL) is a ready-to-use ELISA kit for analyzing human AGRP levels with a detection range of 15.6-1000 pg/mL and a sensitivity of ≤5 pg/mL.
Background:
AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.
Formula Weight:
14,440 Da
Assay Principle:
This experiment uses the double sandwich ELISA technique, and the ELISA kit provided is a typical double sandwich ELISA kit. The pre-coated antibody is a human AGRP monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and biotin-labeled antibodies are added to the ELISA plate wells and washed with PBS or TBS. After the reactants are washed with PBS or TBS, they are colored with TMB substrate, which turns blue when catalyzed by the enzyme peroxidase, and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.
ELISA Type:
Quantitative sandwich
Assay Target:
AGRP
Target Species:
Human
Research Area:
Hormone research; Eating behavior; Signaling pathway; Obesity
Sample Type:
Human serum; Plasma; Cell culture supernatant
Detection Range:
15.6-1000 pg/mL
Sensitivity:
≤5 pg/mL
Precision (Intra-assay):
CV≤8%
Precision (Inter-assay):
CV≤12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human agouti-related protein (AGRP) ELISA kit (15.6-1000 pg/mL, ≤5 pg/mL) is designed for the in vitro quantitative analysis of AGRP levels in human serum, plasma, and cell culture supernatant samples.
Storage:
Store at 2-8°C.