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Human adrenocorticotropic hormone (ACTH) ELISA kit (15.625-1000 pg/mL)
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Cat. No.:
OB0525WXX-179
Synonyms:
Human ACTH ELISA kit; Human adrenocorticotropic hormone (ACTH); Human adrenocorticotropic hormone; Adrenocorticotropic hormone (ACTH); ACTH ELISA kit; Human ACTH
Size:
48T; 96T
Specification
Description:
Human adrenocorticotropic hormone (ACTH) ELISA kit (15.625-1000 pg/mL) is a ready-to-use ELISA kit for analyzing human ACTH levels with a detection range of 15.625-1000 pg/mL and a sensitivity of 5.24 pg/mL.
Background:
ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.
Formula Weight:
26,707 Da
Assay Principle:
The assay utilizes a competitive inhibitor enzyme immunoassay technique. Monoclonal antibodies specific for ACTH are pre-coated on microtiter plates. Biotin-labeled ACTH and unlabeled ACTH (standards or samples) undergo a competitive inhibition reaction with the pre-coated ACTH-specific antibody. After incubation, unbound conjugates are washed away. Next, avidin bound to HRP is added to each microtiter plate well and incubated. The amount of bound HRP is inversely proportional to the concentration of ACTH in the sample. Upon addition of the substrate solution, the intensity of color development is inversely proportional to the concentration of ACTH in the sample.
ELISA Type:
Competitive inhibition
Assay Target:
ACTH
Target Species:
Human
Research Area:
Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
15.625-1000 pg/mL
Sensitivity:
5.24 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human adrenocorticotropic hormone (ACTH) ELISA kit (15.625-1000 pg/mL) is designed for the in vitro quantitative analysis of ACTH levels in human serum, plasma, and other biological fluid samples.
Storage:
Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).