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Human adiponectin (ADP) ELISA kit (62.5-4000 pg/mL, <15 pg/mL)
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Cat. No.:
OB0525WXX-216
Synonyms:
Human ADP ELISA kit; Human adiponectin (ADP); Human adiponectin; Adiponectin (ADP); Human ADP; ADP ELISA kit
Size:
96T
Specification
Description:
Human adiponectin (ADP) ELISA kit (62.5-4000 pg/mL, <15 pg/mL) is a ready-to-use ELISA kit for analyzing human ADP levels with a detection range of 62.5-4000 pg/mL. Its sensitivity is less than 15 pg/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,414 Da
Assay Principle:
The assay utilizes a 96-well plate coated with an antibody specific for human ADP. Standards and samples are pipetted into the wells, and the ADP in the sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-human ADP antibody is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, TMB substrate solution is added to the wells, and the color is developed proportionally to the amount of ADP bound. The termination solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
ELISA Type:
Sandwich
Assay Target:
ADP
Target Species:
Human
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Cell culture supernatants; Serum; Plasma; Tissue homogenates
Detection Range:
62.5-4000 pg/mL
Sensitivity:
<15 pg/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Stability:
12 Months
Applications:
Human adiponectin (ADP) ELISA kit (62.5-4000 pg/mL, <15 pg/mL) is designed for the in vitro quantitative analysis of ADP levels in human cell culture supernatants, serum, plasma, and tissue homogenate samples.
Storage:
Store at 2-8°C.