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Human adiponectin (ADP) ELISA kit (1.56-100 ng/mL, 1.102 ng/mL)
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Cat. No.:
OB0525WXX-261
Synonyms:
Human ADP ELISA kit; Human adiponectin (ADP); Human adiponectin; Adiponectin (ADP); Human ADP; ADP ELISA kit
Size:
24T; 48T; 96T
Specification
Description:
Human adiponectin (ADP) ELISA kit (1.56-100 ng/mL, 1.102 ng/mL) is a ready-to-use ELISA kit for analyzing human ADP levels with a detection range of 1.56-100 ng/mL and a sensitivity of 1.102 ng/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,414 Da
Assay Principle:
The microtiter plates provided in this kit are pre-coated with an ADP-specific antibody. Standards or samples are then added to the appropriate microplate wells along with a biotin-conjugated ADP-specific polyclonal antibody preparation, and HRP-conjugated avidin is then added to each microplate well and incubated. TMB substrate solution is then added to each well. Only wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The addition of sulfuric acid solution terminates the enzyme-substrate reaction, and the color change is then measured spectrophotometrically at 450 nm ± 2 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to the standard curve.
Assay Target:
ADP
Target Species:
Human
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Serum; Plasma; Cell culture supernates; Tissue homogenates; Urine
Detection Range:
1.56-100 ng/mL
Sensitivity:
1.102 ng/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<10%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human adiponectin (ADP) ELISA kit (1.56-100 ng/mL, 1.102 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in human serum, plasma, cell culture supernates, tissue homogenates, and urine samples.
Storage:
Store at 2-8°C.