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Human adiponectin (ADP) ELISA kit (0.78-50 μg/mL)
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Cat. No.:
OB0525WXX-207
Synonyms:
Human ADP ELISA kit; Human adiponectin (ADP); Human adiponectin; Adiponectin (ADP); Human ADP; ADP ELISA kit
Size:
48T; 96T
Specification
Description:
Human adiponectin (ADP) ELISA kit (0.78-50 μg/mL) is a ready-to-use ELISA kit for analyzing human ADP levels with a detection range of 0.78-50 μg/mL. It is an efficient dynamic research tool.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,414 Da
Assay Principle:
The assay utilizes a two-site sandwich ELISA for the quantitative detection of ADP in human serum and plasma. An antibody specific for ADP is pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any ADP will bind to the immobilized antibody. After removing unbound material, a biotin-conjugated antibody specific for ADP is added to the wells. After washing, streptavidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color is developed in proportion to the amount of ADP bound in the first step. The color development is stopped, and the color intensity is measured.
ELISA Type:
Quantitative sandwich
Assay Target:
ADP
Target Species:
Human
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
serum; Plasma; Other biological fluids
Detection Range:
0.78-50 μg/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human adiponectin (ADP) ELISA kit (0.78-50 μg/mL) is designed for the in vitro quantitative analysis of ADP levels in human serum, plasma, and other biological fluid samples.
Storage:
Store at 2-8°C.