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Human adiponectin (ADP) CLIA kit
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Cat. No.:
OB0125WXX-029
Synonyms:
ADP CLIA kit; Human ADP; Human adiponectin (ADP); Adiponectin (ADP)
Size:
24T; 48T; 96T
Specification
Description:
Human adiponectin (ADP) CLIA kit is a CLIA kit assay for the detection of ADP levels. The range of the assay is 13.7-10000 pg/mL.
Background:
Adiponectin is involved in important adipokines that control fat metabolism and insulin sensitivity. It has multiple functions, including antagonizing TNF-α by negatively regulating its expression in different tissues such as liver and macrophages and counteracting its effects; stimulating AMPK phosphorylation and activation in liver and skeletal muscle to enhance glucose utilization and fatty acid burning, and inhibiting endothelial NF-kappa-B signaling through a cAMP-dependent pathway. It is found that it may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,414 Da
Assay Type:
Double-antibody sandwich
Assay Principle:
The microtiter plates in this kit are pre-coated with an antibody specific for ADP. Standards or samples are then added to the appropriate microtiter plate wells, followed by the biotin-conjugated antibody specific for ADP. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. A mixture of substrates A and B is then added to produce glow light emission kinetics. After the microtiter plate is developed, the intensity of the glow is proportional to the level of ADP in the sample or standard.
Assay Target:
ADP
Target Species:
Human
Research Area:
Hormone research; Lipid metabolism; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids
Detection Range:
13.7-10,000 pg/mL
Sensitivity:
5.1 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human adiponectin (ADP) CLIA kit is used to detect ADP levels in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluid samples by chemiluminescent immunoassay.
Storage:
Store at 2-8°C.