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Human acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.781-50 ng/mL, 0.469 ng/mL)
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Cat. No.:
OB0525WXX-126
Synonyms:
Human ACP5 ELISA kit; Human acid phosphatase 5, tartrate resistant (ACP5); Human acid phosphatase 5, tartrate resistant; ACP5 ELISA kit; Human ACP5; Phosphatase 5, tartrate resistant (ACP5); EC 3.1.3.2
Specification
Description:
Human acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.781-50 ng/mL, 0.469 ng/mL) is a ready-to-use ELISA kit for analyzing human ACP5 levels with a detection range of 0.781-50 ng/mL and a sensitivity of 0.469 ng/mL.
Background:
ACP5 is involved in the dephosphorylation of proteins. Its expression appears to increase in certain pathological states. Mice overexpressing ACP5 have been found to exhibit hyperplastic obesity. There are two phenotypes of this enzyme: 5a and 5b. TRAP 5a binds to preadipocytes and may exert cell number-regulating activity, inducing preadipocyte proliferation/differentiation. TRAP 5b may also induce the proliferation of certain cell types.
Formula Weight:
36,599 Da
Assay Principle:
The kit is based on sandwich ELISA. The anti-human ACP5 antibody is pre-coated on a 96-well plate. A biotin-conjugated anti-human ACP5 antibody is then used as the detection antibody. Standards, test samples, and biotin-conjugated detection antibody are subsequently added to the wells and washed with buffer. HRP-streptavidin is added, and the unbound conjugate is washed away with washing solution. TMB substrate is used to show the HRP enzyme reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The density of yellow color is proportional to the amount of human ACP5 sample captured in the plate. The concentration of human ACP5 can be calculated by reading the absorbance at 450 nm with a microplate reader.
ELISA Type:
Double antibody sandwich
Assay Target:
ACP5
Target Species:
Human
Research Area:
Hormone regulation; Metabolites; Defense response; Inflammatory; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
0.781-50 ng/mL
Sensitivity:
0.469 ng/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.781-50 ng/mL, 0.469 ng/mL) is designed for the in vitro quantitative analysis of ACP5 levels in human serum, plasma, and other biological fluid samples.
Storage:
Store at -20°C (microplate, standard) and 2-8°C (other reagents).