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Human acetyl-CoA carboxylase 2 (ACACB) ELISA kit (1.56-100 ng/mL)
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Cat. No.:
OB0525WXX-028
Synonyms:
Human ACACB ELISA kit; Acetyl-CoA carboxylase 2 (ACACB); ACACB; ACACB ELISA kit; Human ACACB; EC 6.3.4.14
Size:
48T; 96T
Specification
Description:
Human acetyl-CoA carboxylase 2 (ACACB) ELISA kit (1.56-100 ng/mL) is a ready-to-use ELISA kit for analyzing human ACACB levels with a detection range of 1.56-100 ng/mL and a sensitivity of 0.5 ng/mL. This kit has high sensitivity and excellent specificity.
Background:
ACACB (also called ACC2) is a subunit of ACC. ACC is responsible for catalyzing the carboxylation of acetyl-CoA to malonyl-CoA, which is the rate-limiting step in fatty acid synthesis. ACC2 has been found to play an important role in controlling fatty acid oxidation and is a potential target for the treatment of obesity and related diseases.
Formula Weight:
276,541 Da
Assay Principle:
This experiment uses double sandwich ELISA technology. The pre-coated antibody is a monoclonal antibody against human ACACB, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and biotin-labeled antibodies are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells; the reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue when catalyzed by the peroxidase enzyme, and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.
ELISA Type:
Sandwich
Assay Target:
ACACB
Target Species:
Human
Research Area:
Metabolites; Fatty acid biosynthesis; Obesity
Sample Type:
Human Serum; Plasma; Cell culture supernatant; Organizations in the natural and recombinant ACACB concentration
Detection Range:
1.56-100 ng/mL
Sensitivity:
0.5 ng/mL
Precision (Intra-assay):
CV≤8%
Precision (Inter-assay):
CV≤12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human acetyl-CoA carboxylase 2 (ACACB) ELISA kit (1.56-100 ng/mL) is designed for the in vitro quantitative analysis of ACACB levels in human serum, plasma, cell culture supernatant, and natural and recombinant ACACB concentration samples.
Storage:
Store at 2-8°C.