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Human 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA kit (31.25-2000 pg/mL)
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Cat. No.:
OB0525WXX-001
Synonyms:
11 Beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1); Human 11β-HSD1 ELISA kit; 11β-HSD1 ELISA kit; Human 11β-HSD1; Human 11 beta-hydroxysteroid dehydrogenase type 1; 11β-Hydroxysteroid dehydrogenase type 1; EC 1.1.1.146
Size:
48T; 96T
Specification
Description:
Human 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA kit (31.25-2000 pg/mL) is a ready-to-use ELISA kit for analyzing human 11β-HSD1 levels with a detection range of 31.25-2000 pg/mL and a sensitivity of 15.6 pg/mL. This kit has high sensitivity and excellent specificity. This kit has high sensitivity and excellent specificity. This kit has high sensitivity and excellent specificity.
Background:
11β-HSD1 catalyzes the reversible conversion of cortisol to the inactive metabolite cortisone and plays a pathogenic role in obesity and metabolic syndrome. It could be utilized as a potential target for anti-obesity studies.
Formula Weight:
32,401 Da
Assay Principle:
An antibody specific for 11β-HSD1 has been pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any 11β-HSD1 will bind to the immobilized antibody. After removing unbound material, a biotin-conjugated antibody specific for 11β-HSD1 is added to the wells. After washing, streptavidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of 11β-HSD1 bound in the initial step. The color development is stopped, and the color intensity is measured.
ELISA Type:
Quantitative sandwich
Assay Target:
11β-HSD1
Target Species:
Human
Research Area:
Lipid metabolism; Hormone research; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
31.25-2000 pg/mL
Sensitivity:
15.6 pg/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA kit (31.25-2000 pg/mL) is designed for the in vitro quantitative analysis of 11β-HSD1 levels in human serum, plasma, and other biological fluid samples.
Storage:
Store at 2-8°C.