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Human 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA kit (30-9000 ng/L)
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Cat. No.:
OB0525WXX-002
Synonyms:
11 Beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1); Human 11β-HSD1 ELISA kit; 11β-HSD1 ELISA kit; Human 11β-HSD1; Human 11 beta-hydroxysteroid dehydrogenase type 1; 11β-Hydroxysteroid dehydrogenase type 1; EC 1.1.1.146
Size:
48T; 96T
Specification
Description:
Human 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA kit (30-9000 ng/L) is a ready-to-use ELISA kit for analyzing human 11β-HSD1 levels with a detection range of 30-9000 ng/L and a sensitivity of 15.19 ng/L. This kit has high sensitivity and excellent specificity.
Background:
11β-HSD1 catalyzes the reversible conversion of cortisol to the inactive metabolite cortisone and plays a pathogenic role in obesity and metabolic syndrome. It could be utilized as a potential target for anti-obesity studies.
Formula Weight:
32,401 Da
Assay Principle:
The plate has been pre-coated with human 11β-HSD1 antibody. The 11β-HSD1 added to the sample binds to the antibody coated on the wells. Biotinylated human 11β-HSD1 antibody is then added and binds to 11β-HSD1 in the sample. Streptavidin-HRP is then added to bind to the biotinylated 11β-HSD1 antibody. After incubation, unbound streptavidin-HRP is washed out. Substrate solution is then added, and color formation is proportional to the amount of human 11β-HSD1. The reaction is terminated by the addition of an acidic termination solution, and the absorbance is measured at 450 nm.
ELISA Type:
Quantitative sandwich
Assay Target:
11β-HSD1
Target Species:
Human
Research Area:
Lipid metabolism; Hormone research; Obesity
Sample Type:
Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates
Detection Range:
30-9000 ng/L
Sensitivity:
15.19 ng/L
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<10%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Stability:
6 Months
Applications:
Human 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA kit (30-9000 ng/L) is designed for the in vitro quantitative analysis of 11β-HSD1 levels in human serum, plasma, cell culture supernates, cell lysates, and tissue homogenate samples.
Storage:
Store at 2-8°C.