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Human 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1) ELISA kit
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Cat. No.:
OB0525WXX-349
Synonyms:
Human AGPAT1 ELISA kit; Human 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1); Human 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha; 1-Acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1); Human AGPAT1; AGPAT1 ELISA kit
Size:
48T; 96T
Specification
Description:
Human 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1) ELISA kit is a ready-to-use ELISA kit for analyzing human AGPAT1 levels with a detection range of 0.312-20 ng/mL and a sensitivity of 0.06 ng/mL.
Background:
AGPAT1 converts lysophosphatidic acid (LPA) into phosphatidic acid (PA), two phospholipids involved in signal transduction and intracellular lipid biosynthesis.
Formula Weight:
31,717 Da
Assay Principle:
The provided ELISA kit is a typical double sandwich ELISA technique. The pre-coated antibody is human AGPAT1 monoclonal antibody and the detection antibody is biotin-labeled polyclonal antibody. The sample and biotin-labeled antibody are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells; the reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue when catalyzed by peroxidase, and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.
ELISA Type:
Sandwich
Assay Target:
AGPAT1
Target Species:
Human
Research Area:
Lipid metabolism; Signaling pathways; Obesity
Sample Type:
Human serum; Plasma; Cell culture supernatant; Organizations in the natural and recombinant AGPAT1 concentration
Detection Range:
0.312-20 ng/mL
Sensitivity:
0.06 ng/mL
Precision (Intra-assay):
CV≤8%
Precision (Inter-assay):
CV≤12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Human 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1) ELISA kit is designed for the in vitro quantitative analysis of AGPAT1 levels in human serum, plasma, cell culture supernatant, organizations in the natural and recombinant AGPAT1 concentration samples.
Storage:
Store at 2-8°C.