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Histone methyltransferase (HMT) activity/inhibition assay kit (H3K4)
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Cat. No.:
OB0125WXX-046
Synonyms:
Histone methyltransferase (HMT); HMT activity/inhibition assay kit (H3K4)
Size:
96T
Specification
Description:
Histone methyltransferase (HMT) activity/inhibition assay kit (H3K4) is an epigenetic test to determine HMT activity/inhibition (H3K4) that is fast and efficient and is completed in less than 3 hours.
Background:
HMT controls or regulates DNA methylation through chromatin-dependent transcriptional repression or activation. It transfers 1-3 methyl groups from S-adenosyl-L-methionine to lysine and arginine residues of histones. Inhibition of HMT leads to silencing of gene expression, and HMT inhibitors are currently being developed for a variety of therapeutic or experimental applications. SET7/9, SET1, etc., are HMTs that catalyze the methylation of histone H3 lysine 4 (H3K4) in mammalian cells. H3K4 methylation is likely a global epigenetic mark in euchromatin and mediates activated transcription. Assays for HMT activity/inhibition are necessary.
Assay Type:
Activity/inhibition assay
Assay Principle:
Histone substrates are stably captured on the wells of the reagent strips during the assay. HMTase transfers a methyl group from Adomet to the histone H3 substrate, methylating the substrate lysine 4. The methylated histone H3K4 is recognized by high-affinity antibodies. The ratio or amount of methylated H3K4 is proportional to enzyme activity and can be quantified by the HRP-conjugated secondary antibody chromogenic system. HMT activity can then be calculated based on the amount of methylated H3K4 converted by HMTs.
Assay Target:
HMT
Target Species:
Universal
Research Area:
Methylation research; Obesity
Sample Type:
Biological samples
Kit Components:
HM1 (10X wash buffer); HM2 (Histone assay buffer); HM3 (Adomet); HM4 (Biotinylated Substrate, 25 µg/mL); HM5 (HMT standard, 10 µg/mL); HM6 (Capture antibody, 100 µg/mL); HM7 (Detection antibody, 200 µg/mL); HM8 (Developing solution); HM9 (Stop solution); Control enzyme (200 µg/mL); 8 well assay strip (With frame)
Applications:
Histone methyltransferase (HMT) activity/inhibition assay kit (H3K4) is ideally suited for the specialized determination of the activity/inhibition of individual HMTs targeting lysine residues at different sites.
Storage:
Store at -20°C (HM3, HM4, HM5, HM7), room temperature (HM9) and 4°C away from light (others).