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Histone H3K4 methylation assay kit
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Cat. No.:
OB0125WXX-051
Synonyms:
Histone H3K4; Histone H3-K4 methylation assay kit; Histone methylation assay kit; Methylation assay kit
Size:
96T
Specification
Description:
Histone H3K4 methylation assay kit is an epigenetic test that uses a colorimetric assay and is a fast and efficient procedure that can be completed in less than 5 hours.
Background:
A major mechanism for the epigenetic inactivation of genes is the methylation of CpG islands in genomic DNA by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcriptional repression or activation. They transfer 1-3 methyl groups from S-adenosyl-l-methionine to lysine and arginine residues of histones. SET7/9, MLL, and others are histone methyltransferases that catalyze histone H3 lysine 4 (H3K4) methylation in mammalian cells. H3K4 methylation serves as a global epigenetic mark in euchromatin and mediates activated transcription. Measurement of global histone H3-K27 trimethylation is necessary.
Assay Type:
Methylation assay
Assay Principle:
In this assay, histones are stably spotted in the wells. Methylated histone H3K4 is recognized by high-affinity antibodies. The proportion or amount of methylated histone H3K4 can be quantified by the intensity of HRP-conjugated secondary antibody color development.
Assay Target:
H3K4 methylation
Target Species:
Universal
Research Area:
Methylation research; Obesity
Sample Type:
Cell; Tissue
Kit Components:
GD1 (10X lysis buffer); GD2 (Extraction buffer); GD3 (10× wash buffer); GD4 (Histone buffer); GD5 (Blocking buffer); GD6 (Antibody buffer); GD7 (Capture antibody, 100 μg/mL); GD8 (Detection antibody, 400 μg/mL); GD9 (Developing solution); GD10 (Stop solution); Methylated H3K4 control (60 μg/mL); 8-well assay strip (With frame)
Applications:
Histone H3K4 methylation assay kit is intended for the specific detection of global histone H3K4 methylation using a variety of mammalian cells, including fresh and frozen tissues as well as cultured adherent and suspension cells.
Storage:
Store at -20°C (GD8, methylated H3K4 control), 4°C away from light (GD3, GD5, GD7, GD9, strip) and room temperature (others).