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Histone H3-K27 tri-methylation assay kit
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Cat. No.:
OB0125WXX-054
Synonyms:
Histone methylation assay kit; Histone H3-K27; Tri-methylation assay kit
Size:
96T
Specification
Description:
Histone H3-K27 tri-methylation assay kit is an epigenetic test that uses a colorimetric assay to measure histone H3-K27 tri-methylation, making the assay more flexible and efficient.
Background:
Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcriptional repression or activation. They transfer 1-3 methyl groups from S-adenosyl-l-methionine to lysine and arginine residues of histones. G9a and polycomb group enzymes are HMTs that catalyze the methylation of histone H3 at lysine 27 (H3-K27) in mammalian cells. H3-K27 trimethylation is a facultative heterochromatin marker that promotes recruitment of polycomb group proteins for gene silencing. It is also found that increased global H3-K27 trimethylation is associated with pathological processes. Measurement of histone H3-K27 trimethylation is essential.
Assay Type:
Methylation assay
Assay Principle:
In this assay, histones are stably spotted on the bar wells when the kit is used for detection. Trimethylated Histone H3-K27 is recognized by high-affinity antibodies. The proportion or amount of trimethylated histone H3-K27 can be quantified by the intensity of HRP-conjugated secondary antibody color development.
Assay Target:
H3-K27 tri-methylation
Target Species:
Universal
Research Area:
Methylation research; Obesity
Sample Type:
Cell; Tissue
Kit Components:
GT1 (10X lysis Buffer); GT2 (Extraction Buffer); GT3 (10× wash buffer); GT4 (Histone buffer); GT5 (Blocking buffer); GT6 (Antibody buffer); GT7 (Capture antibody, 100 µg/ml); GT8 (Detection antibody, 400 µg/ml); GT9 (Developing solution); GT10 (Stop solution); Tri-methyl H3-K27 control (60 µg/ml); 8 well assay strip (With frame)
Applications:
Histone H3-K27 tri-methylation assay kit is suitable for the specific measurement of global histone H3-K27 trimethylation using a variety of mammalian cells, including cultured adherent cells, fresh and frozen tissues, and suspension cells.
Storage:
Store at -20°C (GT8, tri-methyl H3-K27 control), 4°C away from light (GT5, GT7, GT9, strip) and room temperature (others).