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Histone H3-K27 methylation assay kit
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Cat. No.:
OB0125WXX-053
Synonyms:
Histone methylation assay kit; Methylation assay kit; Histone H3-K27
Size:
96T
Specification
Description:
Histone H3-K27 methylation assay kit is an epigenetic test kit that uses a colorimetric assay to measure histone H3-K27 methylation, which is fast and efficient and is completed in less than 3 hours.
Background:
A major mechanism for the epigenetic inactivation of genes is the methylation of CpG islands in genomic DNA by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcriptional repression or activation. G9a and polycomb group enzymes are HMTs that catalyze the methylation of histone H3 at lysine 27 (H3-K27) in mammalian cells. H3-K27 methylation participates in the silencing of gene expression at nonchromatin loci and, through the formation of polycomb binding sites, mediates heterochromatin formation. Mediate the formation of heterochromatin. Increased overall H3-K27 methylation has also been found to be associated with several pathological processes. Measurement of histone H3-K27 methylation is necessary.
Assay Type:
Methylation assay
Assay Principle:
In this assay, histones are stably spotted on bar wells. Methylated histone H3-K27 is recognized by high-affinity antibodies. The proportion or amount of methylated H3-K27 can be quantified by the HRP-conjugated secondary antibody chromogenic system and is proportional to the chromogenic intensity.
Assay Target:
H3-K27 methylation
Target Species:
Universal
Research Area:
Methylation research; Obesity
Sample Type:
Cell; Tissue
Kit Components:
GG1 (10X lysis buffer); GG2 (Extraction buffer); GG3 (10× wash buffer); GG4 (Histone buffer); GG5 (Blocking buffer); GC6 (Antibody buffer); GC7 (Capture antibody, 100 µg/ml); GC8 (Detection antibody, 400 µg/ml); GC9 (Developing solution); GC10 (Stop solution); Methylated H3-K27 control (60 µg/ml); 8 well assay strip (With frame)
Applications:
Histone H3-K27 methylation assay kit is suitable for the specific measurement of global histone H3-K27 methylation using a variety of mammalian cells, including fresh and frozen tissues, cultured adherent cells, and suspension cells.
Storage:
Store at -20°C (GG8, methylated H3-K27 control), 4°C away from light (GG5, GG7, GG9, strip) and room temperature (others).