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Hamster adrenocorticotropic hormone (ACTH) ELISA kit
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Cat. No.:
OB0525WXX-175
Synonyms:
Hamster ACTH ELISA kit; Hamster adrenocorticotropic hormone (ACTH); Hamster adrenocorticotropic hormone; Adrenocorticotropic hormone (ACTH); ACTH ELISA kit; Hamster ACTH
Size:
48T; 96T
Specification
Description:
Hamster adrenocorticotropic hormone (ACTH) ELISA kit is a ready-to-use ELISA kit for analyzing hamster ACTH levels with a detection range of 0.5-100 ng/L and a sensitivity of 0.23 ng/L.
Background:
ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.
Formula Weight:
26,707 Da
Assay Principle:
Plates have been pre-coated with hamster ACTH antibody. Adrenocorticotropic hormone in the sample is added and binds to the antibody coated on the wells. Biotinylated hamster ACTH antibody is then added and binds to ACTH in the sample. Streptavidin-HRP is then added to bind to the biotinylated ACTH antibody. After incubation, unbound streptavidin-HRP is washed out. The substrate solution is then added, and color is developed in proportion to the hamster ACTH content. The reaction is terminated by the addition of an acidic termination solution, and the absorbance is measured at 450 nm.
ELISA Type:
Quantitative sandwich
Assay Target:
ACTH
Target Species:
Hamster
Research Area:
Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity
Sample Type:
Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates
Detection Range:
0.5-100 ng/L
Sensitivity:
0.23 ng/L
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Stability:
6 Months
Applications:
Hamster adrenocorticotropic hormone (ACTH) ELISA kit is designed for the in vitro quantitative analysis of ACTH levels in hamster serum, plasma, cell culture supernates, cell lysates, and tissue homogenate samples.
Storage:
Store at 2-8°C.