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Hamster adiponectin (ADP) ELISA kit (26,133 Da)
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Cat. No.:
OB0525WXX-213
Synonyms:
Hamster ADP ELISA kit; Hamster adiponectin (ADP); Hamster adiponectin; Adiponectin (ADP); Hamster ADP; ADP ELISA kit
Size:
48T; 96T
Specification
Description:
Hamster adiponectin (ADP) ELISA kit (26,133 Da) is a ready-to-use ELISA kit for analyzing hamster ADP levels with high specificity and sensitivity.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,133 Da
Assay Principle:
The assay uses a two-site sandwich ELISA to quantify ADP in the sample. An antibody specific for ADP is pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any ADP present is bound by the immobilized antibody. After removing unbound material, a biotin-conjugated antibody specific for ADP is added to the wells. After washing, streptavidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color is developed in proportion to the amount of ADP bound in the first step. The color development is stopped, and then the color intensity is measured.
ELISA Type:
Sandwich
Assay Target:
ADP
Target Species:
Hamster
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Sample Volume:
1-200 μL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Hamster adiponectin (ADP) ELISA kit (26,133 Da) is designed for the in vitro quantitative analysis of ADP levels in hamster serum, plasma, and other biological fluid samples.
Storage:
Store at 2-8°C.