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FOXO reporter kit (PI3K/AKT signaling pathway)
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Cat. No.:
OB0125WXX-078
Synonyms:
FOXO reporter; Forkhead reporter; PI3K reporter; AKT reporter; FOXO1 reporter; FOXO3 reporter; FOXO4 reporter; Cell based assay kit; Cell-based assay; Reporter assay kit
Size:
500rxns
Specification
Description:
FOXO reporter kit (PI3K/AKT signaling pathway) is designed to monitor the activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cellular models.
Background:
The PI3K/AKT signaling pathway is critical for cell growth and survival and has been implicated in a variety of diseases, including obesity. The mammalian FOXO proteins (FOXO1, FOXO3, and FOXO4), a subgroup of forkhead transcription factors, are one of the most well-characterized targets of the PI3K/AKT signaling pathway. These transcription factors trigger apoptosis by upregulating genes required for cell death. Insulin or growth factor induces activation of PI3K, which in turn activates AKT. AKT directly phosphorylates FOXOs, leading to the export of FOXOs from the nucleus to the cytoplasm, thereby inhibiting FOXO-dependent transcription.
Research Area:
Signaling pathway research; Obesity
Sample Type:
Biological samples
Kit Components:
Reporter mix (FOXO luciferase reporter vector, constitutively expressing Renilla luciferase vector); Negative control reporter mix (non-inducible luciferase vector, constitutively expressing Renilla luciferase vector); FOXO3 expression vector; Negative control expression vector (empty expression vector, without FOXO3)
Applications:
FOXO reporter kit (PI3K/AKT signaling pathway) is used to monitor PI3K/AKT signaling pathway activity and FOXO transcriptional activity, to study the effect of RNAi or gene overexpression on PI3K/AKT pathway activity, and to screen for compounds affecting PI3K/AKT signaling pathway activity.
Storage:
Store at 2-8°C.