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Duck agouti-related protein (AGRP) ELISA kit
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Cat. No.:
OB0525WXX-367
Synonyms:
Duck AGRP ELISA kit; Duck agouti-related protein (AGRP); Duck agouti-related protein; Agouti-related protein (AGRP); Duck AGRP; AGRP ELISA kit
Size:
48T; 96T
Specification
Description:
Duck agouti-related protein (AGRP) ELISA kit is a ready-to-use ELISA kit for analyzing duck AGRP levels with high sensitivity.
Background:
AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.
Formula Weight:
14,440 Da
Assay Principle:
The kit utilizes a two-site sandwich ELISA for the quantitative detection of AGRP in samples. An antibody specific for AGRP has been pre-coated on a microplate. Standards and samples are pipetted into the wells, and any AGRP is bound by the immobilized antibody. After removing unbound material, a biotin-conjugated antibody specific for AGRP is added to the wells. After washing, streptavidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, substrate solution is added to the wells, and the color is developed in proportion to the amount of AGRP bound in the first step. The color development is stopped, and the intensity of the color is measured.
ELISA Type:
Sandwich
Assay Target:
AGRP
Target Species:
Duck
Research Area:
Hormone research; Eating behavior; Signaling pathway; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Duck agouti-related protein (AGRP) ELISA kit is designed for the in vitro quantitative analysis of AGRP levels in Duck serum, plasma, and other biological fluid samples.
Storage:
Store at 2-8°C.