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Dog adiponectin (ADP) ELISA kit
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Cat. No.:
OB0525WXX-210
Synonyms:
Dog ADP ELISA kit; Dog adiponectin (ADP); Dog adiponectin; Adiponectin (ADP); Dog ADP; ADP ELISA kit
Size:
48T; 96T
Specification
Description:
Dog adiponectin (ADP) ELISA kit is a ready-to-use ELISA kit for analyzing dog ADP levels with a detection range of 0.312-20 ng/mL. Its sensitivity is less than 0.085 ng/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,414 Da
Assay Principle:
The microtiter plate provided in this kit is pre-coated with an antibody specific for the target antigen. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific to the target antigen, and HRP-conjugated avidin is added to each microtiter plate well and incubated. TMB substrate solution is then added to each well. Only wells containing the target antigen, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of the target antigen in the sample is then determined by comparing the OD of the sample to a standard curve.
Assay Target:
ADP
Target Species:
Dog
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates; Cell culture supernates; Other biological fluids
Detection Range:
0.312-20 ng/mL
Sensitivity:
<0.085 ng/mL
Precision (Intra-assay):
CV≤6.6%
Precision (Inter-assay):
CV≤10.1%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Dog adiponectin (ADP) ELISA kit is designed for the in vitro quantitative analysis of ADP levels in dog serum, plasma, tissue homogenates, cell culture supernates, and other biological fluid samples.
Storage:
Store at 2-8°C.