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Chicken adiponectin (ADPN) CLIA kit
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Cat. No.:
OB0125WXX-031
Synonyms:
ADPN CLIA kit; Chicken ADPN; Chicken adiponectin (ADPN)
Size:
24T; 48T; 96T
Specification
Description:
Chicken adiponectin (ADPN) CLIA kit is a CLIA kit assay for the detection of chicken ADPN levels. The range of the assay is 10.97-8000 pg/mL.
Background:
Adiponectin is involved in important adipokines that control fat metabolism and insulin sensitivity. It has multiple functions, including antagonizing TNF-α by negatively regulating its expression in different tissues such as liver and macrophages and counteracting its effects; stimulating AMPK phosphorylation and activation in liver and skeletal muscle to enhance glucose utilization and fatty acid burning, and inhibiting endothelial NF-kappa-B signaling through a cAMP-dependent pathway. It is found that it may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,461 Da
Assay Type:
Double-antibody sandwich
Assay Principle:
The microtiter plates in this kit are pre-coated with an antibody specific for ADPN. Standards or samples are then added to the corresponding microtiter plate wells along with a biotin-conjugated antibody specific for ADPN. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. A mixture of substrates A and B is then added to produce glow emission kinetics. After the microtiter plate is developed, the intensity of the glow is proportional to the level of ADPN in the sample or standard.
Assay Target:
ADPN
Target Species:
Chicken
Research Area:
Hormone research; Lipid metabolism; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
10.97-8,000 pg/mL
Sensitivity:
4.73 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Chicken adiponectin (ADPN) CLIA kit is used to detect chicken ADPN levels in serum, plasma, and other biological fluid samples by chemiluminescent immunoassay.
Storage:
Store at 2-8°C.