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Chicken adiponectin (ADP) ELISA kit (0.625-40 ng/mL, 0.292 ng/mL)
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Cat. No.:
OB0525WXX-252
Synonyms:
Chicken ADP ELISA kit; Chicken adiponectin (ADP); Chicken adiponectin; Adiponectin (ADP); Chicken ADP; ADP ELISA kit
Size:
48T; 96T
Specification
Description:
Chicken adiponectin (ADP) ELISA kit (0.625-40 ng/mL, 0.292 ng/mL) is a ready-to-use ELISA kit for analyzing chicken ADP levels with a detection range of 0.625-40 ng/mL and a sensitivity of 0.292 ng/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,461 Da
Assay Principle:
The microtiter plates provided in this kit are pre-coated with an antibody specific for ADP. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for ADP. Avidin conjugated to HRP is then added to each microplate well and incubated. Upon addition of TMB substrate solution, only the wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to a standard curve.
ELISA Type:
Sandwich
Assay Target:
ADP
Target Species:
Chicken
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
0.625-40 ng/mL
Sensitivity:
0.292 ng/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Chicken adiponectin (ADP) ELISA kit (0.625-40 ng/mL, 0.292 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in chicken serum, plasma, and other biological fluid samples.
Storage:
Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).