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Chicken adiponectin (ADP) ELISA kit (0.125-8 ng/mL)
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Cat. No.:
OB0525WXX-253
Synonyms:
Chicken ADP ELISA kit; Chicken adiponectin (ADP); Chicken adiponectin; Adiponectin (ADP); Chicken ADP; ADP ELISA kit
Size:
24T; 48T; 96T
Specification
Description:
Chicken adiponectin (ADP) ELISA kit (0.125-8 ng/mL) is a ready-to-use ELISA kit for analyzing chicken ADP levels with a detection range of 0.125-8 ng/mL and a sensitivity of 49 pg/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,414 Da
Assay Principle:
The detection principle used in this kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit is pre-coated with an antibody specific for ADP. Standards or samples are then added to the corresponding microtiter plate wells along with the biotin-conjugated ADP-specific antibody. Avidin conjugated to HRP is then added to each microtiter plate well and incubated. Upon addition of the TMB substrate solution, only the wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to a standard curve.
ELISA Type:
Double antibody sandwich
Assay Target:
ADP
Target Species:
Chicken
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
0.125-8 ng/mL
Sensitivity:
49 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Chicken adiponectin (ADP) ELISA kit (0.125-8 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in chicken serum, plasma, and other biological fluid samples.
Storage:
Store at 2-8°C.