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Chicken acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.781-50 ng/mL)
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Cat. No.:
OB0525WXX-116
Synonyms:
Chicken ACP5 ELISA kit; Chicken acid phosphatase 5, tartrate resistant (ACP5); Chicken acid phosphatase 5, tartrate resistant; ACP5 ELISA kit; Chicken ACP5; Phosphatase 5, tartrate resistant (ACP5); EC 3.1.3.2
Size:
24T; 48T; 96T
Specification
Description:
Chicken acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.781-50 ng/mL) is a ready-to-use ELISA kit for analyzing chicken ACP5 levels with a detection range of 0.781-50 ng/mL and a sensitivity of 0.469-50 ng/mL.
Background:
ACP5 is involved in the dephosphorylation of proteins. Its expression appears to increase in certain pathological states. Mice overexpressing ACP5 have been found to exhibit hyperplastic obesity. There are two phenotypes of this enzyme: 5a and 5b. TRAP 5a binds to preadipocytes and may exert cell number-regulating activity, inducing preadipocyte proliferation/differentiation. TRAP 5b may also induce the proliferation of certain cell types.
Formula Weight:
36,599 Da
Assay Principle:
This ELISA kit utilizes the sandwich-ELISA method. The microplates provided in the kit are pre-coated with an antibody specific for ACP5. Standards or samples are added to the corresponding microplate wells and bound to the specific antibody. The biotinylated detection antibody specific for ACP5 and avidin-HRP conjugate are then added sequentially to each microplate well and incubated. The free components are washed away, and substrate solution is added to each well. Only wells containing ACP5, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon addition of sulfuric acid solution, the enzyme-substrate reaction terminates, and the color changes to yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The OD value is proportional to the concentration of ACP5. The concentration of ACP5 in the sample can be calculated by comparing the OD value of the sample with the standard curve.
ELISA Type:
Sandwich
Assay Target:
ACP5
Target Species:
Chicken
Research Area:
Hormone regulation; Metabolites; Defense response; Inflammatory; Obesity
Sample Type:
Serum; Plasma; Biological fluids
Detection Range:
0.781-50 ng/mL
Sensitivity:
0.469 ng/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Chicken acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.781-50 ng/mL) is designed for the in vitro quantitative analysis of ACP5 levels in chicken serum, plasma, and biological fluid samples.
Storage:
Store at 4°C.