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Cellular senescence staining kit
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Cat. No.:
OB0125WXX-066
Synonyms:
Cellular senescence assay kit; Cellular senescence
Size:
50T
Specification
Description:
A cellular senescence staining kit is a cellular senescence assay kit that provides an effective method for visualizing senescence-associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. The results are later visualized with a standard light microscope.
Background:
Cells stop growing during proliferation and develop a senescent phenotype. This limited lifespan correlates with the age of the organism and the expected lifespan of the species acquiring the cells; the older or shorter the lifespan, the less able the cells are to undergo population multiplication. Senescent cells are characterized by irreversible G1 growth arrest, including repression of genes that drive cell cycle progression and up-regulation of cell cycle repressors such as p16INK4a, p53, and its transcriptional target p21CIP1. They are resistant to mitogen-induced proliferation and show a characteristic enlarged, flattened morphology. Therefore, the study of positively regulated pathways of senescence and how cells bypass senescence is crucial for disease development.
Assay Principle:
SA-β-galactosidase catalyzes the hydrolysis of X-gal to produce the blue color. Each kit can be used for 50 assays in 35 mm Petri dishes.
Assay Target:
Cellular senescence staining
Target Species:
Universal
Research Area:
Cellular senescence research; Obesity
Sample Type:
Biological samples
Kit Components:
Staining solution A; Staining solution B; Staining solution C; Staining solution D; 100X Fixing solution; X-gal solution
Applications:
Cellular senescence staining kit is suitable for efficient and rapid detection of senescence in various cell types.
Storage:
Store at -20°C away from light (X-gal solution) and 4°C (others).