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Cellular senescence activity assay kit (96-Well)
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Cat. No.:
OB0125WXX-064
Synonyms:
Cellular senescence assay kit; Cellular senescence
Size:
120T
Specification
Description:
Cellular senescence activity assay kit (96-Well ) is a cellular senescence assay kit that provides a validated method for measuring senescence-associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Senescence is subsequently quantified using a fluorescent plate reader.
Background:
Cells stop growing during proliferation and develop a senescent phenotype. This limited lifespan correlates with the age of the organism and the expected lifespan of the species acquiring the cells; the older or shorter the lifespan, the less able the cells are to undergo population multiplication. Senescent cells are characterized by irreversible G1 growth arrest, including repression of genes that drive cell cycle progression and up-regulation of cell cycle repressors such as p16INK4a, p53, and its transcriptional target p21CIP1. They are resistant to mitogen-induced proliferation and show a characteristic enlarged, flattened morphology. Therefore, the study of positively regulated pathways of senescence and how cells bypass senescence is crucial for disease development.
Assay Principle:
The assay uses cell lysates to measure SA-ß-Gal lactosidase activity and is normalized to samples containing different cell counts.
Assay Target:
Cellular senescence activity
Target Species:
Universal
Research Area:
Cellular senescence research; Obesity
Sample Type:
Biological samples
Kit Components:
2X Reaction buffer; 2X Cell lysis buffer; SA-ß-Gal substrate (20X); Stop solution
Applications:
Cellular senescence activity assay kit (96-Well) is suitable for detecting cellular senescence and has the advantages of sensitivity and high efficiency.
Storage:
Store at -20°C (SA-ß-Gal substrate) and room temperature (others).