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Cattle adrenocorticotropic hormone (ACTH) ELISA kit (12.35-1000 pg/mL)
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Cat. No.:
OB0525WXX-163
Synonyms:
Cattle ACTH ELISA kit; Cattle adrenocorticotropic hormone (ACTH); Cattle adrenocorticotropic hormone; Adrenocorticotropic hormone (ACTH); ACTH ELISA kit; Cattle ACTH
Size:
24T; 48T; 96T
Specification
Description:
Cattle adrenocorticotropic hormone (ACTH) ELISA kit (12.35-1000 pg/mL) is a ready-to-use ELISA kit for analyzing cattle ACTH levels with a detection range of 12.35-1000 pg/mL. Its sensitivity is less than 4.57 pg/mL.
Background:
ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.
Formula Weight:
26,707 Da
Assay Principle:
This assay uses a competitive inhibition enzyme immunoassay technique. Monoclonal antibodies specific for ACTH are pre-coated on a microtiter plate. Biotin-labeled ACTH and unlabeled ACTH (standards or samples) undergo a competitive inhibition reaction with the pre-coated ACTH-specific antibody. After incubation, unbound conjugates are washed away. Next, avidin bound to HRP is added to each microtiter plate well and incubated. The amount of bound HRP is inversely proportional to the concentration of ACTH in the sample. Upon addition of the substrate solution, the intensity of color development is inversely proportional to the concentration of ACTH in the sample.
ELISA Type:
Competitive inhibition
Assay Target:
ACTH
Target Species:
Cattle
Research Area:
Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity
Sample Type:
Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids
Detection Range:
12.35-1000 pg/mL
Sensitivity:
<4.57 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Cattle adrenocorticotropic hormone (ACTH) ELISA kit (12.35-1000 pg/mL) is designed for the in vitro quantitative analysis of ACTH levels in cattle serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples.
Storage:
Store at 2-8°C.