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Cat interleukin 6 (IL-6) ELISA kit (2-600 ng/L)
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Cat. No.:
OB0125WXX-045
Synonyms:
IL-6 ELISA kit; Cat IL-6; Interleukin 6 (IL-6); Cat IL-6 ELISA kit
Size:
48T; 96T
Specification
Description:
Cat interleukin 6 (IL-6) ELISA kit (2-600 ng/L) is a ready-to-use microwell, strip plate ELISA kit for analyzing the presence of cat IL-6 in the range of 2-600 ng/L.
Background:
IL-6 is a cytokine with multiple biological functions. It is a potent inducer of the acute phase response, acting on T cells, B cells, hematopoietic progenitors, hepatocytes, and cells of the central nervous system. It plays an important role in the eventual differentiation of B cells into Ig-secreting cells. It also acts as an actin, which is excreted into the bloodstream after muscle contraction and can promote lipolysis and improve insulin resistance.
Formula Weight:
23,759 Da
Assay Principle:
The plate has been pre-coated with cat IL-6 antibody. IL-6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated cat IL-6 Antibody is added and binds to IL-6 in the sample. Then streptavidin-HRP is added and binds to the biotinylated IL-6 antibody. After incubation, unbound streptavidin-HRP is washed away during a washing step. Substrate solution is then added, and color develops in proportion to the amount of cat IL-6. The reaction is terminated by the addition of acidic stop solution, and absorbance is measured at 450 nm.
ELISA Type:
Quantitative sandwich
Assay Target:
IL-6
Target Species:
Cat
Research Area:
Inflammatory research; Cytokine research; Obesity
Sample Type:
Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates
Detection Range:
2-600 ng/L
Sensitivity:
1.36 ng/L
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<12%
Applications:
Cat interleukin 6 (IL-6) ELISA kit (2-600 ng/L) is designed for the detection of cat IL-6 in serum, plasma, cell lysates, cell culture supernatants, and tissue homogenates.
Storage:
Store at 2-8°C.