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Cat adiponectin (ADP) ELISA kit (1.25-40 mμg/mL)
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Cat. No.:
OB0525WXX-291
Synonyms:
Cat ADP ELISA kit; Cat adiponectin (ADP); Cat adiponectin; Adiponectin (ADP); Cat ADP; ADP ELISA kit
Size:
48T; 96T
Specification
Description:
Cat adiponectin (ADP) ELISA kit (1.25-40 mμg/mL) is a ready-to-use ELISA kit for analyzing cat ADP levels with a detection range of 1.25-40 mμg/mL and a sensitivity of 0.1 mμg/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,414 Da
Assay Principle:
The wells of the plate are coated with purified bovine ADP antibody to form a solid-phase antibody. Then, ADP and HRP-labeled ADP antibody are added to the wells to form an antibody-antigen-antibody-enzyme complex. After a complete-ish, TMB substrate is added and the TMB substrate turns blue when catalyzed by the enzyme HRP. Then the reaction is terminated by the addition of sulfuric acid, followed by spectrophotometric analysis at 450 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample with the standard curve.
ELISA Type:
Sandwich
Assay Target:
ADP
Target Species:
Cat
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Serum; Plasma; Tissue homogenate; Feces; Urine
Detection Range:
1.25-40 mμg/mL
Sensitivity:
0.1 mμg/mL
Precision (Intra-assay):
CV<15%
Precision (Inter-assay):
CV<15%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Cat adiponectin (ADP) ELISA kit (1.25-40 mμg/mL) is designed for the in vitro quantitative analysis of ADP levels in cat serum, plasma, tissue homogenate, feces, and urine samples.
Storage:
Store at 2-8°C.