Products
Online Inquiry
Canine interleukin 6 (IL-6) ELISA kit (Sandwich)
Inquiry
Cat. No.:
OB0125WXX-043
Synonyms:
IL-6 ELISA kit; Canine IL-6; Interleukin 6 (IL-6); Canine IL-6 ELISA kit
Size:
48T; 96T
Specification
Description:
Canine interleukin 6 (IL-6) ELISA kit (Sandwich) is a ready-to-use microwell, strip plate ELISA kit for analyzing the presence of canine IL-6.
Background:
IL-6 is a cytokine with multiple biological functions. It is a potent inducer of the acute phase response, acting on T cells, B cells, hematopoietic progenitors, hepatocytes, and cells of the central nervous system. It plays an important role in the eventual differentiation of B cells into Ig-secreting cells. It also acts as an actin, which is excreted into the bloodstream after muscle contraction and can promote lipolysis and improve insulin resistance.
Formula Weight:
22,945 Da
Assay Principle:
This assay employs a two-site sandwich ELISA to quantify IL-6 in samples. An antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells, and any IL-6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-6 is added to the wells. After washing, Streptavidin conjugated HRP is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells, and color develops in proportion to the amount of IL-6 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
ELISA Type:
Sandwich
Assay Target:
IL-6
Target Species:
Canine
Research Area:
Inflammatory research; Cytokine research; Obesity
Sample Type:
Serum; Plasma; Other biological fluids
Sample Volume:
1-200 μL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Applications:
Canine interleukin 6 (IL-6) ELISA kit (Sandwich) is designed to measure canine IL-6 in plasma, serum, and other biological fluids.
Storage:
Store at 2-8°C.